Cortisol1Cortisols synthesized from cholesterol and its production Is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. Cortisol plays a role in the metabolism of proteins, lipids, and carbohydrates .It affects blood glucose levels, helps maintain blood pressure and regulate the immune system.

The level of cortisol in the blood normally rises and falls in a “diurnal variation” pattern. It peaks early in the morning, then declines throughout the day. This pattern can change when a person works irregular shifts and sleeps at different times of the day, and it can become disrupted when a disease or condition either limits or stimulates cortisol production.


Increased cortisol levels can monitor:

  • Cushing syndrome
  • Adrenal tumors in patients

Decreased cortisol levels can monitor:

  • Underactive or damaged adrenal glands {adrenal insufficiency) that limit cortisol production; this is referred to as primary adrenal insufficiency, known as Addison disease.
  • An underactive pituitary gland or a pituitary gland tumor that Inhibits
  • ACTH production; this Is known as secondary adrenal Insufficiency.
  • Cortisol2

     Serum  cortisol  levelwill  become  abnormal in cushing”s

                                             ACTH Serum Sample

Catalog Number SDC5454
Reagent Pack 100
Principle Automated Chemiluminescence Immunoassay(CLIA)
Sensitivity 3 pg/mL
Dynamic range  3.0-2000 pg/mL
Sample Type Serum
Intra-assay precision 4.84%
Inter-assay precision 8.52%
Sample Volume 200 μL

SDi CX ACTH vs. SIEMENS Immulite 2000

A comparison of SIEMENS Immulite 2000 ACTH with SDi CX ACTH using clinical samples gave the following correlations (pg/mL):

y = 0.934x + 1.179

R² = 0.976