The Lysozyme ELISA provides researchers with a sensitive assay to measure levels of lysozyme in human samples. This direct sandwich ELISA assay can detect lysozyme from 50ng/ml down to 0.75 ng/mL
Lysozyme is one of the anti-microbial agents present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears and is also found in human milk. Lysozyme has 130 amino acids and its natural substrate is the bacterial cell wall peptidoglycan. Since synthesized by granulocytes and macrophages, lysozyme can act as a useful marker for myelomonocytic cells. Increased levels of lysozyme in urine and serum are diagnostic indicators for acute monocytic leukemia and acute myelomonycytic leukemia. Elevated lysozyme levels were found in synovial fluids of the inflammatory arthritides and osteoarthritis. Salivary lysozyme, a marker for oral infection and hyperglycemia, might display a significant relationship with hypertension, an early stage of cardiovascular disease. Increased serum lysozyme activity is also present in tuberculosis, sarcoidosis, megaloblastic anemias, acute bacterial infections, ulcerative colitis and Crohns disease. Elevated lysozyme levels in urine and serum occur during severe renal insufficiency, renal transplant rejection, urinary tract infections, glomerulonephritis and nephrosis.
Principle of the Assay
The SDi Lysozyme ELISA kit is designed for detection of Lysozyme in human plasma, serum, or stool. This assay employs a quantitative sandwich enzyme immunoassay technique that measures Lysozyme in 75 minutes. A monoclonal antibody specific for Lysozyme has been pre-coated onto a microplate. Lysozyme in standards and samples is sandwiched by the immobilized antibody and an HRP-conjugate monoclonal detection antibody specific for Lysozyme. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
Run time: 75min
Dynamic range: 0.75 – 50ng/ml
Sample type: Serum, Plasma, Stool
Sample volume: 25ul